Johannes Debler

Johannes Wolfram Debler

Molecular Plant Pathologist & Fungal Genomicist

Centre for Crop and Disease Management · Curtin University · Perth, Australia

About

I am a molecular plant pathologist and fungal genomicist based in Perth, Western Australia. My research focuses on the Ascochyta pathogens of cool-season grain legumes, particularly lentil and chickpea, where I investigate the molecular mechanisms of fungal pathogenicity through effector biology and comparative genomics.

My PhD at Curtin University's Centre for Crop and Disease Management (2021–2025) centred on effector discovery in Ascochyta lentis and Ascochyta rabiei. This work led to new tools for the research community, including a boscalid selectable marker system and new telomere-to-telomere (T2T) genome assemblies for two pathotypes of Ascochyta lentis.

Beyond the bench, I am passionate about science communication, demonstrated by winning FameLab Australia 2024, and about mentoring the next generation of researchers.

11 Peer-reviewed publications
16+ Conference presentations
4 Awards
10+ Years at Curtin University

Education

2021–2025
PhD — Molecular Plant Pathology Curtin University, Perth, Australia Effector discovery in Ascochyta pathogens of lentil and chickpea
2011
Diplom (M.Sc.) — Molecular Human Genetics University of Bremen, Germany Expression analysis of selected microRNAs in malignant tumors
2009
Certificate of Proficiency University of Auckland, New Zealand Semester abroad — Forensic Science, Bioinformatics, Structural Biology

Publications

Full list on ORCiD 0000-0002-3604-051X

Protocols

I also share lab protocols openly on protocols.io, including methods for high-molecular-weight DNA extraction, quick fungal DNA extraction, GateWay cloning prep, and Agrobacterium electroporation.

Presentations & Conferences

Awards & Recognition

Experience

Professional

2025-present
Research Associate Curtin University — CCDM, Perth

Molecular cloning, Oxford Nanopore sequencing, fungal transformation, bioinformatic analysis of fungal genomes, effector candidate selection.

2014–2025
Research Assistant Curtin University — CCDM, Perth

Molecular cloning, Oxford Nanopore sequencing, fungal transformation, bioinformatic analysis of fungal genomes, effector candidate selection.

2014
Research Assistant Ear Science Institute of Australia, Perth

Microarray expression data analysis; immunostaining of rat tympanic membrane slides.

2014
Work Experience Forensic Science Centre — University of Western Australia

DNA extraction and PCR assay development for bovine embryo sexing; porcine mitochondrial DNA analysis.

2013
Laboratory Assistant Odyssey Consulting, Bunbury

Immunological assays including quantitative ELISAs for bovine IgG, BVDV antigen and tetracycline concentration.

2012–2013
Tissue Culture Specialist Serana (WA), Bunbury

GMP/ISO 9001 quality assurance, endotoxin testing, SDS-PAGE/western blot, tissue culture media production.

2011
Laboratory Technician Practice for Human Genetics, Bremen

Molecular biological analysis of patient samples (PCR, Sanger sequencing).

2010–2011
Research Assistant Centre for Human Genetics — University of Bremen

RNA/DNA extraction from FFPE tissue, cDNA preparation, PCR and qRT-PCR.

Volunteer & Community

2026-present
Committee Member — Combined Biological Sciences Meeting Co-convenor

Responsible for organising and managing the conference.

2020–2025
Committee Member — Combined Biological Sciences Meeting Trade Coordinator

Responsible for the trade exhibition area of the annual conference.

2023–2025
NTEU Workplace Representative Curtin University

Advocate for the National Tertiary Education Union within the local Curtin community.

2019–2023
Membership Officer — Professionals at Curtin (P@C) Curtin University

Developing opportunities for all Curtin staff to enhance knowledge, skills and experience.

2017–2023
STEM Educator iFLY Indoor Skydiving, Perth

Teaching Year 5–11 students the physics of gravity and drag using wind tunnel demonstrations.

2008–2011
Gymnastics Trainer University of Bremen, Germany

Trainer and supervisor running the university gymnastics group.

Professional Memberships

  • AusBiotech
  • Australian Society for Biochemistry and Molecular Biology (ASBMB)
  • Australian Society for Microbiology (ASM)

Contact

I am always happy to discuss research, collaboration opportunities, or science communication.